基本説明
Contains a detailed protocol chapter which serves as a lab manual with the most important methods for DNA extraction, PCR and electrophoretic analysis of degraded samples.
Full Description
This is the definitive source of information on techniques for the identification and sequencing of old DNA (pieces) and their use in biological and medical research and application. Application of aDNA techniques are useful tools for investigations reaching from evolutionary studies to law enforcement approaches. What brings them together is the interest in specific methods of handling aDNA, i.e. elaborated PCR and sequencing techniques and the interpretation of the results. This books serves as an ideal guideline for it demonstrates how problem-solving strategies can be applied in various areas.
Contents
1 Introduction.- 2 DNA markers.- 3 aDNA extraction.- 4 PCR.- 5 Analysis of PCR products.- 6 Authenticity of results.- 7 Applications.- 8 Protocols.- I Laboratory equipment.- I.1 Pre-PCR laboratory equipment.- I.2 PCR laboratory.- I.3 Post-PCR laboratory.- I.4 Photographic documentation.- II PCR trouble shooting.- III Internet sites of interest.