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基本説明
Contents: DNA arrays for expression measurement: a historical perspective; Expression profiling with cDNA microarrays: a user's perspective and guide; cDNA microarrays on nylon with colourimetric detection;...
Full Description
This complete and practical manual on expression measurement using DNA arrays covers the existing methods (from nylon macroarrays to oligonucleotide chips) and includes detailed protocols. It has been written by practising scientists who have experienced the difficulties involved in actually using microarrays, and provides helpful advice and hints on setting up these powerful but sometimes tricky methods. Software, data mining procedures and probable future developments, which should be useful to any practising scientist interested in expression measurement, are also covered in this book. It also provides detailed protocols as well as many helpful hints to achieve experimental success and to avoid pitfalls.
Contents
DNA arrays for expression measurement: a historical perspective; Expression profiling with cDNA microarrays: a user's perspective and guide; cDNA microarrays on nylon with colourimetric detection; cDNA macroarrays and microarrays on nylon membrames with radioactive detection; Oligonucleotide chips for expression analysis: principles and practical procedures; Gene expression data mining and analysis; Future trends in the use of DNA arrays for expression measurement.