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基本説明
Contents: Generation of recombinant antibodies from hybridoma; Generation of antibody libraries; Isolation of antibody fragments from combinatorial libraries; and more.
Full Description
Interest in recombinant antibody technologies has rapidly increased because of the wide range of possible applications in therapy and diagnosis, especially in cancer treatment. The possibility of generating human antibodies that are not accessible by conventional polyclonal or monoclonal approaches has forced the development of antibody engineering technologies even more. This manual presents a comprehensive collection of detailed, step-by-step protocols provided by experts in the field. All basic methods needed in antibody engineering - not only methods to generate recombinant antibodies, but also protocols for analysis and their use - and recently developed and emerging technologies are covered.
Contents
50 chapters describing protocols in the following areas (Part titles): Introduction * Generation of recombinant antibodies from hybridoma * Generation of antibody libraries * Isolation of antibody fragments from combinatorial libraries * Expression and purification of antibody fragments in bacteria * Expression and purification of antibody fragments in eukaryotic cells * Determination of affinities * Sequence and structure analysis and modelling epitope mapping * In vivo experiments * Affinity maturation of antibody fragments * Humanisation of antibody fragments * Antibody engineering to improve stability * Bivalent and bispecific antibody fragments * Recombinant antibody-fusion proteins * Intracellular targeting of antibody fragments.The complete table of contents can be found on the Internet: http://www.springer.de
