Full Description
The detection of single molecules opens up new horizons in analytical chemistry, biology and medicine. This discipline, which belongs to the expanding field of nanoscience, has been rapidly emerging over the last ten years. This handbook provides a thorough overview of the field. It begins with basics of single molecule detection in solution, describes methods and devices (fluorescense correlation spectroscopy, surface enhanced Raman scattering, sensors, especially dyes, screening techniques, especially confocal laser scanning microscopy). In the second part, various applications in life sciences and medicine provide the latest research results. This modern handbook is a highly accessible reference for a broad community from advanced researchers, specialists and company professionals in physics, spectroscopy, biotechnology, analytical chemistry, and medicine. Written by leading authorities in the field, it is timely and fills a gap - up to now there exists no handbook concerning this theme.
Contents
Obituary.List of Contributors.Single Molecule Detection in Liquids and on Surfaces under Ambient Conditions: Introduction and Historical Overview. Theoretical Foundations of Single Molecule Detection in Solution. Conceptual Basis of Fluorescence Correlation Spectroscopy and Related Techniques as Tools in Bioscience. Surface--enhanced Raman Scattering (SERS) -- A Tool for Single Molecule Detection in Solution. Single Molecule Detection on Surfaces with the Confocal Laser Scanning Microscope. Spectroscopy of Individual Photosynthetic Pigment--Protein Complexes. Single--Molecule Identification in Solution: Principles and Applications. Studying Molecular Motors on the Single Molecule Level. The Chemistry of a Single Enzyme Molecule. Single Molecule Detection of Specific Nucleic Acid Sequences. Single Molecule Detection in the Near--infrared.Name Index.Subject Index.
