基本説明
Contents: Instrumentation and operation of DNA Chromatography; dHPLC; Applications of DNA Chromatography; Collection of material, fragment collection; Data input and output; and more.
Description
(Text)
Die DNA Chromatographie ist eine neue Methode zur Analyse und präparativen Trennung von DNA. Die Vorteile gegenüber der klassischen gel-elektrophoretischen Auftrennung liegen vor allem in der Automatisierbarkeit und der hohen Geschwindigkeit.
Das Werk gibt eine Einführung in die Grundlagen und Anwendungsmöglichkeiten des neuen Verfahrens. Der Text ist auch für diejenigen verständlich geschrieben, die bisher noch nicht chromatographisch gearbeitet haben. Molekularbiologen und Genomforscher in Universität und Industrie erhalten damit das Rüstzeug für das Verständnis und die richtige Umsetzung der neuen Methode im eigenen Labor.
(Table of content)
Introduction
INSTRUMENTATION AND OPERATION
CHROMATOGRAPHIC PRINCIPLES FOR DNA SEPARATION
DHPLC
SIZE BASED SEPARATIONS
PURIFICATION OF NUCLEIC ACIDS
RNA CHROMATOGRAPHY
SPECIAL TECHNIQUES
LOOKING FORWARD
Glossary
System Cleaning and Passivation Treatment
Frequently Asked DHPLC Questions
(Author portrait)
Douglas T. Gjerde received his PhD in analytical chemistry from Iowa State University. After having worked in large and small companies for a number of years, Gjerde founded Sarasep, Inc. in 1990 for the commercialization of polymeric separation technologies and co-founded Transgenomic, Inc. in 1997 for the commercialization of nucleic acid separation technology. Gjerde is now working on his third start-up company, PhyNexus, Inc. PhyNexus produces miniaturized sample preparation tools for the biological research market. Gjerde has authored and coauthored numerous articles, book chapters and several books and holds over fifty patents in separation science.David Hornby studied biochemistry at the University of Sheffield (UK). Since 1999 he is a full professor for Molecular Biology and Biotechnology at the same institution. He has also co-founded two start-up companies (Invector and Protealysis), based on novel technologies for RNA separation and stabilisation, for which he holds the patents. His current research focuses on RNAi biochemistry and proteomics technologies.
