Full Description
This ground-breaking atlas of oocyte ultrastructure forms a practical guide to assisted reproduction. Extensively illustrated with transmission electron microscopic (TEM) images, it contains chapters on oocyte maturation, fertilization, fertilization failure, pitfalls and technical problems of intracytoplasmic sperm injection, and failed fertilization in vitro to explain the principles and evaluation of TEM images. A large chapter of TEM images of the ultrastructure of human oocytes illustrates fertilization failures after ICSI and IVF and the ultrastructure of germinal vesicle oocytes and metaphase I oocytes. It also contains detailed information about abnormal metaphase II oocytes with sections on autolysis, multi-vacuolated mature oocyte, refractile body, and fragmented oocyte. The concluding chapter describes materials and methods for single-cell transmission electron microscopy of the human oocyte.
Contents
List of contributors. Acknowledgements. Preface. Oocyte maturation in vivo and in vitro and assisted reproduction technologies: a review. Normal fertilization. Fertilization failure: the role of the male and female gamete with particular reference to intracytoplasmic sperm injection. The intracytoplasmic sperm injection technique: pitfalls and essentials. Failed fertilization in vitro: principles and evaluation of transmission electron microscopic images. Ultrastructure of human oocytes: a transmission electron microscopic view. Materials and methods for single-cell transmission electron microscopy of the human oocyte. Index.
