工業用細胞培養ハンドブック<br>Handbook of Industrial Cell Culture : Mammalian, Microbial, and Plant Cells

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工業用細胞培養ハンドブック
Handbook of Industrial Cell Culture : Mammalian, Microbial, and Plant Cells

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  • 製本 Hardcover:ハードカバー版／ページ数 536 p.
  • 言語 ENG
  • 商品コード 9781588290328
  • DDC分類 660.6

基本説明

Provides essential technique linking new methods in the fields of molecular genetics, proteomics, genomics, analytical biochemistry and screening for the industrial manipulation and propagation of cells.

Full Description

A diverse team of researchers, technologists, and engineers describe, in simple and practical language, the major current and evolving technologies for improving the biocatalytic capabilities of mammalian, microbial, and plant cells. The authors present state-of-the-art techniques, proven methods, and strategies for industrial screening, cultivation, and scale-up of these cells, and describe their biotech and industrial uses. Special emphasis is given to the solving critical issues encountered during the discovery of new drugs, process development, and the manufacture of new and existing compounds. Other topics include recombinant protein expression, bioinformatics, high throughput screening, analytical tools in biotechnology, DNA shuffling, and genomics discovery.

Contents

Part I. Mammalian Cell Culture

Analysis and Manipulation of Recombinant Glycoproteins Manufactured in Mammalian Cell Culture
Nigel Jenkins

Genetic Approaches to Recombinant Protein Production in Mammalian Cells
Peter P. Mueller, Dagmar Wirth, Jacqueline Unsinger, and Hansjörg Hauser

Protein Expression Using Transgenic Animals
William H. Velander and Kevin E. van Cott

Mammalian Cell Culture: Process Development Considerations
Steven Rose, Thomas Black, and Divakar Ramakrishnan

Part II. Microbial Cell Culture

Natural Products: Discovery and Screening
Matthew D. Hilton

Genetic Engineering Solutions for Natural Products in Actinomycetes
Richard H. Baltz

Culture Medium Optimization and Scale-Up for Microbial Fermentations
Neal C. Connors

Part III. Plant Cell Culture

Functional Genomics for Plant Trait Discovery
Sam Reddy, Ignacio M. Larrinua, Max O. Ruegger, Vipula K. Shukla, and Yuejin Sun

Molecular Tools for Engineering Plant Cells
Donald J. Merlo

Plant Cell Culture: A Critical Tool for Agricultural Biotechnology
Joseph F. Petolino, Jean L. Roberts, and Ponsamuel Jayakumar

Expression of Recombinant Proteins via the Plastid Genome
Jeffrey M. Staub

Oleosin Partitioning Technology for Production of Recombinant Proteins in Oil Seeds
Maurice M. Moloney

Part IV. Critical Tools for Biotechnology

Genome Sequencing and Genomic Techniques in Drug Discovery and Development
Lawrence M. Gelbert

Proteomics
Gerald W. Becker, Michael D. Knierman, Pavel Shiyanov, and John E. Hale

Metabolic Flux Analysis, Modeling, and Engineering Solutions
Walter M. van Gulik, Wouter A. van Winden, and Joseph J. Heijnen

Advances in Analytical Chemistry for Biotechnology: Mass Spectrometry of Peptides, Proteins, andGlycoproteins
Jeffrey S. Patrick

DNA Shuffling for Whole Cell Engineering
Steve del Cardayre and Keith Powell

Cell Culture Preservation and Storage for Industrial Bioprocesses
James R. Moldenhauer

Index