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Full Description
This text puts into perspective the plant tissue culture requirements for particular applications within the plant sciences and enables students to undertake experiments with minimal guidance.
Contents
Introduction and techniques: introductory history; laboratory organization; media; aseptic manipulation. Basic aspects: cell culture; cellular totipotency; somatic embryogenesis. Applications to plant breeding: haploid production; triploid production; in vitro pollination and fertilization; zygotic embryo culture; somatic hybridisation and cybridisation; genetic transformation; somaclonal and gametoclonal variant selection. Application to horticulture and forestry: production of disease-free plants; clonal propagation. General applications: industrial applications - secondary metabolite production; germplasm conservation.