Enzyme Kinetics and Mechanisms

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Enzyme Kinetics and Mechanisms

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  • 製本 Hardcover:ハードカバー版／ページ数 244 p.
  • 言語 ENG
  • 商品コード 9781402007286
  • DDC分類 572.744

基本説明

Takes the reader through the experimental techniques and the logic by which the mechanisms of enzyme-catalyzed reactions can be elucidated by the results of steady-state kinetics and related experiments.

Full Description

Enzyme Kinetics and Mechanisms takes the reader through the experimental techniques and the logic by which the mechanisms of enzyme-catalyzed reactions can be elucidated by the results of steady-state kinetics and related experiments. It is meant to make these investigations both satisfying and effective. In distinction to other available descriptions, the descriptions in enzyme Kinetics and Mechanisms are limited to more commonly utilized and useful models and techniques. The logic relating the chemical models to the mathematical models and the logic of relating the mathematical models to data is presented in rather concise text, figures and equations. The development of mathematical models from chemical models is done by a unique algorithm that is both simple and quick, and the same concept are utilized to develop models for the effects of a variety of reaction conditions on the initial velocity. In addition, the various relationships of data, mathematical models and the chemical models is illustrated with examples from the scientific literature.

Contents

Steady-State Kinetics.- The Generation of Experimental Data.- Methods for Model Evaluation.- Derivation of Mathematical Models.- The Effect of Substrate Concentration.- Effects of Analog Inhibitors.- Effects of Product Inhibitors.- Effects of Substrate Inhibition.- Slow and Tight Inhibition.- The Thermodynamics of Initial Velocity.- Effects of PH.- Effects of Isotopic Substitution.- Effects of other Reaction Conditions.