Biopolymer Methods in Tissue Engineering (Methods in Molecular Biology Ser)

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Biopolymer Methods in Tissue Engineering (Methods in Molecular Biology Ser)

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  • 製本 Hardcover:ハードカバー版/ページ数 400 p.
  • 言語 ENG
  • 商品コード 9780896039674
  • DDC分類 610.28

Full Description

There is an urgent need to develop new approaches to treat conditions as- ciated with the aging global population. The surgeon's approach to many of these problems could be described as having evolved through three stages: Removal: Traditionally, diseased or badly damaged tissues and structures might simply be removed. This was appropriate for limbs and non-essential organs, but could not be applied to structures that were critical to sustain life. An additional problem was the creation of disability or physical deformity that in turn could lead to further complications. Replacement: In an effort to treat wider clinical problems, or to overcome the limitations of amputation, surgeons turned to the use of implanted materials and medical devices that could replace the functions of biological structures. This field developed rapidly in the 1960s and 1970s, with heart valve and total joint replacement becoming common. The term "biomaterial" was used increasingly to describe the materials used in these operations, and the study of biomaterials became one of the first truly interdisciplinary research fields. Today, biomaterials are employed in many millions of clinical procedures each year and they have become the mainstay of a very successful industry.

Contents

Processing of Resorbable Poly-a-Hydroxy Acids for Use as Tissue-Engineering Scaffolds
Minna Kellomäki and Pertti Törmälä

Fibrin Microbeads (FMB) As Biodegradable Carriers for Culturing Cells and for Accelerating Wound Healing
Raphael Gorodetsky, Akiva Vexler, Lilia Levdansky, and Gerard Marx

Synthesis and Characterization of Hyaluronan-Based Polymers for Tissue Engineering
Carlo Soranzo, Davide Renier, and Alessandra Pavesio

Synthesis and Characterization of Chitosan Scaffolds for Cartilage-Tissue Engineering
Steven H. Elder, Dana L. Nettles, and Joel D. Bumgardner

Characterization of a Calcium Phosphate-Based Matrix for rhBMP-2
Hyun D. Kim, John M. Wozney, and Rebecca H. Li

Methodologies for Processing Biodegradable and Natural Origin Scaffolds for Bone and Cartilage Tissue-Engineering Applications
Manuela E. Gomes, Patrícia B. Malafaya, and Rui L. Reis

Alginates in Tissue Engineering
Marcy Wong

Production and Surface Modification of Polylactide-Based Polymeric Scaffolds for Soft-Tissue Engineering
Yang Cao, Tristan I. Croll, Justin J. Cooper-White, Andrea J. O'Connor, and Geoffrey W. Stevens

Modification of Materials With Bioactive Peptides
Jennifer L. West

Isolation and Osteogenic Differentiation of Bone-Marrow Progenitor Cells for Application in Tissue Engineering
António J. Salgado, Manuela E. Gomes, Olga P. Coutinho, and Rui L. Reis

Cell Seeding of Polymer Scaffolds
Gordana Vunjak-Novakovic and Milica Radisic

Chondrocyte Isolation, Expansion, and Culture on Polymer Scaffolds
Aileen Crawford and Sally C. Dickinson

Bioreactor Culture Techniques for Cartilage-Tissue Engineering
David A. Lee and Ivan Martin

Microscopic Methods for the Analysis of Engineered Tissues
Sally Roberts and Janis Menage

Transmission Electron Microscopy ofTissue-Polymer Constructs
Paul V. Hatton

Application of Microscopic Methods for the Detection of Cell Attachment to Polymers
John Hunt and Deborah Heggarty

Biochemical Methods for the Analysis of Tissue-Engineered Cartilage
Wa'el Kafienah and Trevor J. Sims

Real-Time Quantitative RT-PCR Assays
Ivan Martin and Oliver Frank

Mechanical Testing of Cell-Material Constructs: A Review
John Kisiday, Alex Kerin, and Alan Grodzinsky

Index