Full Description
In Leukemia and Lymphoma: Detection of Minimal Residual Disease, hands-on experts describe and discuss the minimal residual disease (MRD) methods they have successfully pioneered for leukemias and lymphomas. They apply reverse transcription PCR (RT-PCR) to acute myeloid leukemia (AML), chronic myelogenous leukemia (CML), and acute promyelocytic leukemia (APL). Other PCR methods are used for Non-Hodgkin's Lymphoma and for the monitoring of follicular lymphoma. Additional chapters address the use of real-time quantitative PCR (RQ-PCR), the emergent method of choice, in patients with acute lymphoblastic leukemia (ALL), the evaluation of MRD techniques in clinical trials, and the application of flow cytometry techniques.
Contents
1 Flow-Cytometry Methods for the Detection of Residual Leukemia.- 2 Flow-Cytometry—Based Studies of Minimal Residual Disease in Children with Acute Lymphoblastic Leukemia.- 3 PCR Methods for the Detection of Minimal Residual Disease in Childhood Acute Lymphoblastic Leukemia.- 4 Statistical Considerations in the Analysis of Minimal Residual Disease.- 5 The Application of Minimal Residual Disease Detection Methodology to the Clinical Decision-Making Process.- 6 Measurement of Minimal Residual Disease in Children Undergoing Allogeneic Stem Cell Transplant for Acute Lymphoblastic Leukemia.- 7 Molecular Investigation of Minimal Residual Disease in Adult Acute Lymphoblastic Leukemia of B Lineage.- 8 Investigation of Minimal Residual Disease in Acute Myeloid Leukemia by Immunophenotyping.- 9 Minimal Residual Disease in Acute Myeloid Leukemia: RT-PCR—Based Studies of Fusion Transcripts.- 10 Minimal Residual Disease in Acute Promyelocytic Leukemia.- 11 Minimal Residual Disease in Chronic Myelogenous Leukemia: Results of RT-PCR Detection of BCR-ABL Transcripts.- 12 Utility of PCR Assessment of Minimal Residual Disease After Stem Cell Transplantation in Non-Hodgkin's Lymphoma.- 13 Real-Time Polymerase Chain Reaction of Genomic DNA for Quantitation of t(14;18).- 14 Monitoring Follicular Lymphoma by Polymerase Chain Reaction.- 15 Editors' Notes.
