Full Description
Histotechnology and histomorphometry are the major methodologies in bone and cartila- related research. Handbook of Histology Methods for Bone and Cartilage is an outgrowth of the editors' own quest for information on bone and cartilage histology and histomorphometry. It is designed to be an experimental guide for personnel who work in the areas of basic and clinical bone and cartilage, orthopedic, or dental research. It is the first inclusive and organized reference book on histological and histomorphometrical techniques on bone and cartilage specimens. The topic has not previously been covered adequately by any existing books in the field. Handbook of Histology Methods for Bone and Cartilage has six major parts and is designed to be concise as well as inclusive, and more practical than theoretical. The text is simple and straightforward. Large numbers of tables, line drawings, and micro- or macro-photographs, are used to help readers better understand the content. Full bibliographies at the end of each chapter guide readers to more detailed information. A book of this length cannot discuss every method for bone and cartilage histology that has been used over the years, but it is hoped that major methods and their applications have been included.
Contents
Part I. Introduction
Introduction to Experimental Bone and Cartilage Histology
Yuehuei H. An and Helen E. Gruber
Part II. Structure and Function of Bone and Cartilage
Cell Structure and Biology of Bone and Cartilage
William R. Walsh, Mark Walton, Warwick Bruce, Yan Yu, Ronald M. Gillies, and Martin Svehla
Normal Structure and Function of Bone
Torben Steiniche and Ellen M. Hauge
Structure and Function of Articular Cartilage
Jerry C. Y. Hu and Kyriacos A. Athanasiou
Part III. Tissue Harvesting, Fixation, and Preparation
Bone-Labeling Techniques
Reinhold G. Erben
Human Bone Biopsy
Robert S. Weinstein
Biopsy Issues for Bone and Cartilage Tumors
John L. Eady
Tissue Harvesting and Fixation
Linda L. Jenkins and Karen J. L. Burg
Common Fixatives in Hard-Tissue Histology
Antonio Scarano, Giovanna Iezzi, and Adriano Piattelli
Decalcification of Bone Tissue
Robert A. Skinner
Principles of Embedding and Common Protocols
Yuehuei H. An, Patricia L. Moreira, Qian K. Kang, and Helen E. Gruber
Infiltration Techniques and Results in Different Types of Resin
Antonio Scarano, Giovanna Orsini, and Adriano Piattelli
Part IV. Sectioning Techniques
Histological Techniques for Decalcified Bone and Cartilage
Qian K. Kang, Jackie C. LaBreck, Helen E. Gruber, and Yuehuei H. An
Techniques for Sectioning Undecalcified Bone Tissue Using Microtomes
William L. Ries
Cutting and Grinding Methods for Hard-Tissue Histology
Thomas W. Bauer and Diane Mahovlic
Bone Sectioning Using the Exakt System
Karl Donath and Michael Rohrer
Bone Sectioning Using a Modified Inner Diamond Saw
Joop G. C. Wolke, Jan-Paul C. M. van der Waerden, Christel P. A. T. Klein, and John A. Jansen
Bone Sectioning Using the Precise 1 Automated Cutting System
Antonio Scarano, Manlio Quaranta, and Adriano Piattelli
Bone, Cement, and Metal Implant Interface Preparation with a High-Pressure Water Cutter
Jian-Sheng Wang and Lars Lidgren
Part V. Staining Techniques
Basic Staining and Histochemical Techniques and Immunohistochemical Localizations Using Bone Sections
Helen E. Gruber and Jane A. Ingram
Basic Staining Techniques for Cartilage Sections
Helen E. Gruber and Jane A. Ingram
Histochemical and Immunohistochemical Staining of Cartilage Sections
Andreas G. Nerlich
Staining Techniques for Plastic-Embedded Specimens
Antonio Scarano, Giovanna Petrone, and Adriano Piattelli
In Situ Hybridization of Bone and Cartilage
Shintaro Nomura and Seiichi Hirota
Part VI. Analysis Techniques
Bone Histomorphometry: Concepts and Common Techniques
Jean E. Aaron and Patricia A. Shore
Histological Analysis of Bone-Implant Interface
John A. Jansen
Histomorphometric Analysis of Bone-Cement and Cement-Metal Interface
Jian-Sheng Wang, Gonzalo G. Valdivia, Michael J. Dunbar, Cecil H. Rorabeck, Robert B. Bourne, and Suzanne Maher
Histologic Analysis of Bone Healing
Ryland B. Edwards, III, Mandi J. Lopez, and Mark D. Markel
Histomorphometry of Metabolic Bone Conditions
Ellen M. Hauge, Torben Steiniche, and Troels T. Andreassen
Histology of Articular Cartilage Repair
Stefan Nehrer and Myron Spector
Histological Analysis of Cartilage Conditions
Theodore R. Oegema, Jr., Cathy S. Carlson, and Ada A. Cole
Histological Analysis of Soft-Tissues Biomaterial Interface: Relevance to Dental Implants
Antonio Scarano, Gian Antonio Favero, Elisabetta Fiera, and Adriano Piattelli
Pathological Diagnosis of Common Tumors of Bone and Cartilage
Jasvir S. Khurana and Kri
