Transgenic Mouse : Methods and Portocols (Methods in Molecular Biology)

Transgenic Mouse : Methods and Portocols (Methods in Molecular Biology)

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  • 製本 Hardcover:ハードカバー版／ページ数 3741 p.
  • 言語 ENG
  • 商品コード 9780896039155
  • DDC分類 616.93

Full Description


Marten Hofker and Jan van Deursen have assembled a multidisciplinary collection of readily reproducible methods for working with mice, and particularlyfor generating mouse models that will enable us to better understand gene function. Described in step-by-step detail by highly experienced investigators, these proven techniques include new methods for conditional, induced knockout, and transgenic mice, as well as for working with mice in such important research areas as immunology, cancer, and atherosclerosis. Such alternative strategies as random mutagenesis and viral gene transduction for studying gene function in the mouse are also presented.

Contents

IntroductionModification of the Mouse: General Technology; Pronuclear and Blastocyst Injection. In Vitro Fertilization/Cryopreservation. Transgene Design. Generating Conditional Mouse Mutants via Tetracycline-Controlled Gene Expression. Manipulating Large Insert Clones for Transgenesis. Reporter Molecules in Genetically Engineered Mice. Gene Targeting in Mouse Embryonic Stem Cells. Conditional Knockout Mice. Knockin Approaches. Aggregation of Embryos and Embryonic Stem Cells. Andenovirus-Mediated Gene Transfer. Random ENU Mutagenesis. Autopsy and Histologic Analysis of the Transgenic Mouse. Bone Marrow Transplantations to Study Gene Function in Hematopoietic Cells. Quantification of Atherosclerosis in Mice. Genetically Modified Mice in Cancer Research. Neuropathobiology in Transgenic Mice: The Case of Alzheimer's Disease. Index.