基本説明
Significantly updates the best-selling First edition.
Full Description
In this fully updated and revised 2nd edition of "Gene Therapy Protocols", leading experts from academic and industrial laboratories around the world detail their most effective viral and nonviral methods of gene transfer, as well as discuss their applications in different organ systems. These methods range from those in which new molecular conjugates show great promise for targeting gene transfer and regulating transgene expression, to those used in such exciting applications as the delivery of therapeutic proteins, vaccination, and tissue engineering. Up-to-date and highly practical, "Gene Therapy Protocols, 2nd Edition", offers a rich compilation of the revolutionary advances that have recently occurred in gene transfer technology, with each article providing proven the step-by-step laboratory procedures that enable its successful therapeutic application.
Contents
Contents Agarose and Polyacrylamide Gel Electrophoresis Andrea M. Guilliatt Internal Labeling of DNA Probes Ralph Rapley and Bimal D. M. Theophilus End-Labeling of DNA Probes Adrian J. Harwood Southern Blotting of Agarose Gels by Capillary Transfer Ralph Rapley and Ian J. Williams Restriction Fragment Length Polymorphism Mohammad S. Enayat PCR: Principles, Procedures, and Parameters Nicola Louise Jones Allele-Specific Oligonucleotide PCR Elaine K. Green Long-Range PCR, Peter A. Davies and George Gray Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing Anu Wartiovaara and Ann-Christine Syvanen Cycle Sequencing of PCR Products G. K. Surdhar Fluorescent In Situ Hybridization Sara A. Dyer and Elaine K. Green. The Protein Truncation Test Carol A. Hardy Mutation Detection in Factor VIII cDNA from Lymphocytes of Haemophilia A Patients by Solid Phase Fluorescent Chemical Cleavage of Mismatch Naushin H. Waseem, Richard Bagnall, Peter M. Green, and Francesco Giannelli Denaturing Gradient Gel Electrophoresis Yvonne Wallis Conformation-Sensitive Gel Electrophoresis Ian J. Williams and Anne C. Goodeve SSCP/Heteroduplex Analysis Andrew J. Wallace Cleavase(R) Fragment Length Polymorphism Analysis for Genotyping and Mutation Detection Laura Heisler and Chao-Hung Lee Automated Genotyping Using the DNA MassArray(tm) Technology Christian Jurinke, Dirk van den Boom, Charles R. Cantor, and Hubert Koster An Introduction to Bioinformatics Henry Brzeski
