Capillarty Electrophoresis of Carbohydrates (Methods in Molecular Biology Vol 213)

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Capillarty Electrophoresis of Carbohydrates (Methods in Molecular Biology Vol 213)

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  • 製本 Hardcover:ハードカバー版/ページ数 306 p.
  • 言語 ENG
  • 商品コード 9780896038264
  • DDC分類 572

Full Description

Glycoconjugates such as glycoproteins and glycolipids play important roles in cell-cell interaction events, including development, differentiation, m- phogenesis, fertilization, inflammation, and metastasis. A number of reports have documented the association of unique oligosaccharide sequences to p- tein targeting and folding, and in mechanisms of infection, inflammation, and immunity. For glycoproteins, these glycan appendages are the result of extensive co- or post-translational modifications of the nascent proteins in the endoplasmic reticulum and in the Golgi apparatus. Although nucleic acids and proteins are copied from a template in a repeated series of identical steps using the same enzymes, complex carbohydrates are formed by the sequential actions of cellular glycosyltransferases that specifically recognize unique s- strates. The molecular biology of these transferases and other carbohydra- modifying enzymes is providing important insights on oligosaccharide recognition events.
While it is acknowledged that the definition of the protein complement of cells and tissues (the so-called proteome) remains an enormous task in this postgenomic era, the characterization of all glycans produced by individual organisms (referred to as the glycome) presents an equally imp- tant challenge. This task is further complicated by the fact that oligosacc- rides cannot presently be cloned. These complex carbohydrates exist in a staggering diversity of structures, linkages, and branching, thus providing an exquisite molecular repertoire for cellular interactions.

Contents

Part I. Introduction

Structural and Functional Diversity of Glycoconjugates: A Formidable Challenge to the Glycoanalyst
Gerald W. Hart

Part II. Sample Preparation Methods

Chemical and Enzymatic Release of Glycans from Glycoproteins
Tony Merry and Sviatlana Astrautsova

Derivatization of Carbohydrates
Shigeo Suzuki, John F. Kelly, Steven J. Locke, Pierre Thibault, and Susumu Honda

Preparation of Oligosaccharides from Sulfated Glycosaminoglycans Using Bacterial Enzymes
Shuhei Yamada and Kazuyuki Sugahara

Part III. Analysis of Mono- and Oligosaccharides

Monosaccharide Analysis by Capillary Electrophoresis
Susumu Honda

Separation of Oligosaccharides by Capillary Electrophoresis Using Buffer Modifiers
Susumu Honda and Shigeo Suzuki

Characterization of Oligosaccharides from Starch, Dextran, Cellulose, and Glycoproteins by Capillary Electrophoresis
Fu-Tai A. Chen

Affinity Capillary Electrophoresis of Oligosaccharides
Atsushi Taga and Susumu Honda

Characterization of Glycosaminoglycans by Capillary Electrophoresis
Robert J. Linhardt and Toshihiko Toida

Part IV. Analysis of Glycoconjugates and the Component Monosaccharides

Analysis of 2-Aminoacridone-Derivatized Complex Oligosaccharides Using Micellar Electrokinetic Capillary Chromatography and Laser-Induced Fluorescence Detection
Helen C. Birrell and Patrick Camilleri

Separation of Protein Glycoforms by Capillary Electrophoresis
Myriam Taverna, Nguyet Thuy Tran, and Danielle Ferrier

Separation of Glycoproteins by Capillary Isoelectric Focusing
Ira S. Krull, Sarah Kazmi, Huijuan Zhong, and Ling Chen Santora

Glycoprotein Analysis by Capillary Electrophoresis-Electrospray Mass Spectrometry
Kevin P. Bateman, John F. Kelly, Pierre Thibault, Louis Ramaley, and Robert L. White

Analysis of Bacterial Glycolipids by Capillary Electrophoresis-Electrospray Mass Spectrometry: Haemophilus influenzae and Neisseria meningitidis Lipopolysaccharides
Pierre Thibault, Adèle Martin, Michel Gilbert, Warren W. Wakarchuk, and James C. Richards

Part V. Specific Applications

Capillary Electrophoresis as an Assay Method for Monitoring Glycosyltransferase Activity
Warren W. Wakarchuk and Anna-Maria Cunnningham

Determination of Association Constant of Carbohydrate-Protein Interaction
Atsushi Taga and Susumu Honda

Appendix

Structures of Carbohydrates Found in Animals and Bacteria
Shigeo Suzuki, Malcolm Perry, Pierre Thibault, and Susumu Honda

Index