Full Description
Stephen P Robinson, MD, PhD, and Andrew Stagg, PhD, have brought together a wide range of time-proven methods for studying these dendritic cells. Many of these readily reproducible techniques deal with the problem of obtaining sufficient dendritic cells for analysis, whether by isolation from a wide vareity of tissues, or from various progenitor cell populations. Other methods describe in step-by-step fashion the techniques commonly used for analyzing aspects of dendritic cells, ranging from cell migration to antigen uptake and T cell stimulation. In addition, a few techniques explore the practical challenges involved in using dendritic cells in a clinical setting to develop novel immunotherapeueutics.
Contents
Part I. Isolation of Dendritic Cells from Animal Tissues Isolation of Dendritic Cells from Mouse Lymph Nodes Dmitry Gabrilovich Isolation of Mouse Spleen Dendritic Cells Andrew J. Stagg, Fiona Burke, Suzanne Hill, and Stella C. Knight Isolation of Mouse Thymic Dendritic Cells Fabienne Anjuere and Carlos Ardavin Isolation of Dendritic Cells from Rat Intestinal Lymph and Spleen Gordon MacPherson, Michelle Wykes, Fang-Ping Huang, and Chris Jenkins Isolation, Enrichment, and Culture of Murine Epidermal Langerhans Cells Franz Koch, Eckhart Kampgen, Gerold Schuler, and Nikolaus Romani Murine Intestinal Dendritic Cell Isolation Paul Pavli Identification and Isolation of Rodent Lung Dendritic Cells Philip A. Stumbles, Jennifer Thomas, and Patrick G. Holt Isolation and Propagation of Mouse Liver-Derived Dendritic Cells Lina Lu, Maureen L. Drakes, and Angus W. Thomson Part II. Isolation and Characterization of Human Dendritic Cells Identification and Immunophenotypic Analyses of Peripheral Blood Dendritic Cells Stephen P. Robinson Purification of Dendritic Cells from Peripheral Blood Steven Patterson, Aaron Rae, and Heather Donaghy Isolation of Human Tonsillar Dendritic Cells Paul U. Cameron and Gianna Stent Isolation of Dendritic Cells from Human Afferent Lymph Christoph U. Brand, Nikhil Yawalkar, Robert E. Hunger, and Lasse R. Braathen Isolation of Human Skin Dendritic Cells by In Vitro Migration Cornelia D. Richters, Matthias J. Hoekstra, John S. du Pont, Robert W. Kreis, and Eduard W. A. Kamperdijk Human Intestinal Dendritic Cell Isolation Paul Pavli Isolation of Human Lung Dendritic Cells Michel M. van den Heuvel, Carin E. G. Havenith, and Robert H. J. Beelen Identification and Isolation of Synovial Dendritic Cells Allison R. Pettit, Lois Cavanagh, and Ranjeny Thomas Part III. Derivation of Animal Dendritic Cells from Progenitor Cells Generation of Murine Bone-Marrow-Derived Dendritic Cells Asher Maroof Generation of Dendritic Cells from Rat Bone Marrow Timothy Powell, James R. Major, and Gordon MacPherson Derivation of Murine Dendritic Cells from Thymic Precursors Karen Lucas, Dolores Saunders, and Li Wu Part IV Generation of Murine Dendritic Cell Lines Generation of Mouse Dendritic Cell Lines Stefania Citterio, Maria Rescigno, Maria Foti, Francesca Granucci, Malgosia Matyszak, Giampiero Girolomoni, and Paola Ricciardi-Castagnoli Generation of Langerhans Cell-Like Dendritic Cells from Murine Fetal Skin Thilo Jakob and Mark C. Udey Derivation of Dendritic Cell Lines from Mouse Skin Akira Takashima and Paul R. Bergstresser Part V. Generation of Human Dendritic Cells from Progenitor Cells Propagation of Human Dendritic Cells In Vitro Christophe Caux and Bertrand Dubois Generation of Dendritic Cells from Lymphoid Precursors Anne Galy Dendritic Cell Generation from Highly Purified CD14+ Monocytes Winfried F. Pickl, Otto Majdic, and Walter Knapp Generation of Blood-Derived Human Dendritic Cells for Antitumor Immunotherapy Michel F. Gilliet and Fank O. Nestle Part VI. Aspects of Dendritic Cell Function Chemotaxis of In Vitro Cultured Human Dendritic Cells Walter Luini, Giovanna D'Amico, Sergio Bernasconi, Paola Allavena, Alberto Mantovani, and Silvano Sozzani Adhesion of Dendritic Cells to Endothelia K. Alun Brown Transendothelial Migration and Reverse Transmigration of In Vitro Cultured Human Dendritic Cells Giancarlo Bianchi, Giovanna D'Amico, Silvano Sozzani, Alberto Mantovani, and Paola Allavena In Vivo Assays of Langerhans Cell Migration, Marie Cumberbatch Rebecca J. Dearman, and Ian Kimber In Vitro Migration of Rat Dendritic Cells Kenjiro Matsuno and Taichi Ezaki Antigen Uptake by Dendritic Cells Christophe Caux and Bertrand Dubois Uptake of Antigen-Antibody Complexes by Human Dendritic Cells Neil A. Fanger, Paul M. Guyre, and Robert F. Graziano Immunoelectron Microscopy of Antigen Processing in Dendritic Cells Monique J. Kleijmeer, George Posthuma, and Hans J. Geuze Purification and Characterization of MHC Class II Containing Organelles in Mouse Bone-Marrow-Derived Dendritic Cells Philippe Pierre Detection of Intracellular Cytokines in Dendritic Cells Peter Kelleher Adenoviral-Vector-Mediated Gene Transfer to Dendritic Cells Wenru Song and Ronald G. Crystal Stimulation of Anti-HIV-1 Cytotoxic T Lymphocytes by Dendritic Cells Xiaoli Huang, Zheng Fan, Lian Zheng, and Charles R. Rinaldo, Jr
