基本説明
Covers basic principles, dosage information, sample preparation procedures, analytical instrumentation, and necessary mathematical methods.
Full Description
The use of stable isotopes in nutritional studies is now widespread, and the technique is becoming increasingly popular. Practical applications are numerous and include:calcium and iron absorption studiesstudies looking at the impacts of diet, physical activity, aging, and medical therapy and supplementation on nutrient metabolismthe measurement of energy cost of pregnancystudies on the causes of growth faltering in infantsinvestigations into childhood and adult obesity.This book is designed as a laboratory handbook of methods used to perform stable isotope studies in humans. It covers basic principles, dosage information, sample preparation procedures, analytical instrumentation, and necessary mathematical methods and provides the fundamentals to enable researchers to evaluate and establish stable isotope methods in their own laboratories.
Contents
1: Introduction, S A Abrams and W W Wong 2: The measurement of protein kinetics with stable isotope tracers, F Jahoor, Baylor College of Medicine, USDA/ARS Children's Nutrition Research Center, Houston, Texas,USA 3: Stable isotope studies of macromineral metabolism: Calcium, magnesium and iron, S A Abrams 4: Evaluation of trace mineral status and bioavailability using stable isotopes (zinc, copper, selenium, molybdenum), N M Lowe, University of Central Lancashire, Preston, UK and C S Broome, University of Liverpool, UK 5: Energy utilization with doubly labelled water (2H218O), W W Wong 6: Body composition measurements with 2H218O isotope dilution, W W Wong 7: Stable isotopes and gas chromatoraphy - Mass spectrometry in studies of glucose and metabolism in children, A L Sunehag, and M W Haymond, Baylor College of Medicine, USDA/ARS Children's Nutrition Research Center, Houston, Texas, USA 8: Cholesterol and other lipid metabolism, R E Ostlund, Washington University School of Medicine, St Louis, Missouri, USA 9: Index