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基本説明
Together with high pressure liquid chromatography, capillary electrophoresis and tandem mass spectrometry, electrospray-ionization mass spectrometry (ESI-MS) is presented as an attractive tool and method.
Full Description
Interpreting Protein Mass Spectra is a hands-on laboratory guide for a wide range of researchers investigating the structures of proteins and peptides. The focus is on the interpretation of structural information gathered through electrospray ionization-mass spectrometry (ESI-MS). The book will also provide useful background and protocols for anyone using matrix-assisted laser desorption/ionization (MALDI), fast atom bombardment (FAB), and secondary ion mass
spectrometry (SIMS). The book includes numerous practical examples, detailed discussions of experimental setups, and valuable hints for troubleshooting both methods and the handling of materials.
Contents
1: Electrospray Ionization as a Vehicle for Protein Mass Spectra
2: Analyte Separation Methods: High-Pressure Liquid Chromatography and Capillary Electrophoresis
3: The Isotope Effect at High Mass
4: Calculation of the Analyte Mass
5: Analytical Figures of Merit for a High-Mass Isotope Envelope
6: Isotope Distribution of a Multiply Charged Mass Spectral Peak
7: Experimental Parameters Affecting Electrospray Ionization Mass Spectra
8: The Cation Effect on Electrospray Ionization Mass Spectra
9: Interpretation of Peptide and Protein Mass Spectra
10: Interpretation of Phosphoprotein Mass Spectra
11: Immunological Applications: Proteins and Phosphoproteins
12: Interpretation of Glycoprotein Mass Spectra
Appendix 1
Appendix 2
Appendix 3
Glossary
Index
