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Full Description
Bioanalytical Chemistry provides a thorough introduction for students and practitioners with a broad range of backgrounds from chemistry to medicine. In so doing, it brings together many of the techniques commonly used by biochemists and molecular biologists. The text includes entire chapters on design and implementation of enzyme assays; mass spectrometry; and validation of new methods. Each chapter progresses from basic concepts to applications involving real samples, and ends with a set of problems, while an appendix contains selected answers. The authors have limited mathematical derivations to those that are essential for an understanding of each method and they include a list of suggested reading for further information. This textbook provides an ideal companion for students, researchers, and industrial scientists working in chemistry, biology, biochemistry, pharmacy, and medicine.
Contents
Preface.
Acknowledgments.
1. Spectroscopic Methods for Matrix Characterization.
2. Enzymes.
3. Quantitation of Enzymes and Their Substrates.
4. Immobilized Enzymes.
5. Antibodies.
6. Quantitative Immunoassays with Labels.
7. Biosensors.
8. Directed Evolution for the Design of Macromolecular Bioassay Reagents.
9. Principles of Electrophoresis.
10. Applications of Zone Electrophoresis.
11. Isoelectric Focusing.
12. Capillary Electrophoresis.
13. Centrifugation Methods.
14. Chromatography of Biomolecules.
15. Mass Spectrometry of Biomolecules.
16. Validation of New Bioanalytical Methods.
Suggested References.
References.
Answers to Problems.
Index.
