Full Description
There are numerous books on cellular and molecular protocols for general use in cell biology but very few are exclusively devoted to neurobiology. This book fills this gap and explains in a clear and consistent manner, some of the more commonly used protocols in neuroscience research. Each chapter is written by either the person who invented the procedure or an expert in the field. The format is uniform: "Overview," "Background," "Protocols," and "results and discussion." Each protocol begins with the principle of the technique, studies in cell culture, materials and reagents, and, lastly, step-by-step outline of the procedure itself. This highly practical book is also well illustrated (with 17 four color plates) to make the concepts and procedures easy to understand and perform.
Contents
Analyses of Intracellular Signal Transduction Pathways in CNS Cells * Confocal and Electron Microscopic Tracking of Internalized Neuropeptide/Receptor Complexes * Transfection Methods for Neurons in Primary Culture * Polyethylenimine: a Versatile Cationic Polymer for Plasmid-based Gene Delivery in the CNS * Trasfection of GABAA Receptor with GFP-Tagged Subunits in Neurons and HEK293 Cells * Neuronal Transfection Using Particle-Mediated Gene Transfer * Analysis of Gene Expression in Genetically Labeled Single Cells * Immunocytochemistry and In Situ Hybridization: Interest of their Combinations for Cytofunctional Approaches of Central and Peripheral Neurons * In Situ Reverse Transcriptase PCR for Detection of mRNA in the CNS * Immunocytochemical Labeling Methods and Related Techniques for Ultrastructural Analysis of Neuronal Connectivity * Combined electrophysiological and morphological analysis of CNS neurons * Tract-Tracing Methods at the Light Microscopy Level * Tract-tracing Methods at the Ultrastructural Level * In vivo Analysis of Cell Proliferation and Apoptosis in the CNS * Confocal Imaging of Nerve Cells and their Connections * Confocal Imaging of Calcium Signaling in Cells from Acute Brain Slices
