Essential Cell Biology (2-Volume Set) : A Practical Approach (Practical Approach Series) 〈001〉

Essential Cell Biology (2-Volume Set) : A Practical Approach (Practical Approach Series) 〈001〉

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  • 製本 Hardcover:ハードカバー版/ページ数 304 p.
  • 言語 ENG
  • 商品コード 9780199638307
  • DDC分類 571.6

基本説明

1 Touhey and Heenan: Cell structure, 2 Rietdorf et al: Basic light microscopy, 3 Lucocq: Electron microscopy in cell biology, 4 Hodel and Edwardson: Subcellular Fractionations, 5 Ceriotti et al: Plant Cell Biology, and more.

Full Description


Biological and medical research relies upon an integrated understanding of the molecules within cells and of the interactions between cells. This has imposed great demands on investigators. Being an expert in a relatively narrow area is no longer sufficient as many studies now require the use of a wide range of techniques to provide the necessary integration. A lack of familiarity with the experimental possibilities can make such diversification difficult to achieve. This two volume set of Essential Cell Biology is designed to help researchers overcome these problems. It has not been possible to include all of the techniques available in cell biology so the challenge was to identify those that might be most relevant to researchers who are new to this topic. We have tried to cover both traditional and more recent approaches. The theory and basic principles of each technique are described, together with detailed protocols and advice for trouble shooting. Directions to more specialised techniques are also included.We hope the result inspires readers to experience the challenges and rewards of cell biology research for themselves and to contribute to the ongoing task of understanding the life of the cell. Essential Cell Biology volume 1 focuses on techniques for studying cell structure whilst volume 2 concentrates on understanding how the cell functions. Volume 1 details the essential background information and protocols for observing and understanding cell morphology and cell structure, including, for example, investigations of nucleic acids, lipids, and the cytoskeleton. This is the essential guide to cell biology for researchers new to the field.

Contents

1. Cell structure ; 2. Basic light microscopy ; 3. Electron microscopy in cell biology ; 4. Subcellular Fractionations ; 5. Plant Cell Biology ; 6. Protein Purification ; 7. Gel Electrophoresis of Proteins ; 8. Biophysical methods in structural cell biology ; 9. Nucleic acids ; 10. Cell lipids: from isolation to funational dynamics ; 11. Extracellular matrix protocols for the study of complex phenotypes ; 12. The cytoskeleton