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基本説明
Pregnancy tests, HIV tests and tests to confirm heart attacks and many other clinical diagnostic tests are all immunoassays. This book help any life scientist develop his/her own immunoassay.
Full Description
Immunoassays is the first practical volume designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, for preparing radioactive, enzymatic, fluorescent and other labels, are described. Solid-phase reagents, standards and immunogens are explained, with procedures for their preparation. But most components can be obtained commercially and comprehensive lists of suppliers are given. Approaches to combining the reagents and optimizing the required assay are described, including how to eliminate common interferences, and thorough validation procedures are given. Manual and computer methods for the calculation of concentrations are explained. Since only assays that are within control give valid results, a graded and comprehensive approach to internal and external quality assurance methods is detailed, including how to prepare control samples.
Contents
Analysis by specific binding ; Raising antibodies ; Choosing and characterizing antibodies ; Labels and endpoints ; Solid-phase reagents ; Standard and immunogens ; Optimization ; Validation ; Data processing ; Quality assurance
