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Full Description
For freshman/sophomore courses in General Introductory Microbiology, and Microbiology for Allied Health Students. This laboratory manual serves as a general introduction to the microbiology laboratory, including basic procedures and equipment. Content covers not only basic lab instructions, but background material that explains the tests, the basis of results and their applications, providing a "procedural" aspect in addition to laboratory guidance. Questions are provided with each exercise to reinforce and challenge students' understanding of basic concepts and to require students to analyze or apply the material under discussion.
Contents
I. MICROSCOPIC TECHNIQUES. 1. Introduction to Microscopy. 2. Preparation of Smears and Simple Stains. 3. Wet Mounts and Hanging Drops. 4. The Negative Stain. 5. Differential Staining Procedures: Gram Stain, Acid-Fast Stain. 6. Additional Differential Staining Procedures: Metachromatic-Granules, Capsule, Endospore and Flagella Stains. II. MICROBIAL DIVERSITY. 7. Ubiquity of Microorganisms. 8. Survey of Microorganisms. III. MICROBIAL CULTIVATION TECHNIQUES. 9. Isolation Techniques: Streak Plate. 10. Isolation Techniques: Pour Plates. 11. Oxygen Requirements of Microorganisms. 12. Physical Growth Requirements. 13. Selective and Differential Media. 14. Physiological Characteristics of Bacteria: Carbohydrate Metabolism. 15. Physiologic Characteristics of Bacteria: Reactions of Nitrogen Metabolism. 16. Physiologic Characteristics of Bacteria: Miscellaneous Reactions. 17. Multiple Test Media. IV. IDENTIFICATION TECHNIQUES. 18. Development and Use of Diagnostic Keys. 19. Use of Diagnostic Keys: Bacterial Unknowns. 20. Rapid Diagnostic Tests: Profile Analysis. V. MICROBIAL GROWTH. 21. Microbial Population Counts: Microscopic Methods. 22. Microbial Population Counts: Viable Cell Counts. VI. MICROBIAL CONTROL. 23. Physical Control Methods. 24. UV Light as an Antimicrobial Agent. 25. Assay of Antimicrobial Agents: Disc-Diffusion Methods. 26. Assay of Antimicrobial Agents: Use-Dilution Methods. 27. Antibiotic Sensitivity Testing: The Kirby-Bauer Procedure. 28. Detection of Mutant Strains of Bacteria. VII. CLINICAL MICROBIOLOGY. 29. Epidemiology. 30. Serological Reactions. 31. The Collection and Transport of Clinical Specimen. 32. Throat Cultures. 33. Urinary Tract Cultures. 34. Gastrointestinal Tract Cultures. 35. Lactobacillus Activity. VIII. VIROLOGY. 36. Viral Population Counts: Plaque Counting and Plaque Morphology. IX. APPLIED MICROBIOLOGY. 37. Food Microbiology. 38. Water Analysis: Standard Methods. 39. Water Analysis: Membrane Filter Techniques. Appendix A: Writing a Lab Report. Appendix B: Cultures Used. Appendix C: Media, Stains, Reagents, and Supplies.
