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Full Description
This is the classic resource for undergraduate microbiology laboratory courses just keeps getting better. The self-contained, clearly illustrated exercises and four-color format make Benson's "Microbiological Applications: A Laboratory Manual in General Microbiology" the ideal lab manual. Appropriate for either a majors or non-majors lab course, Benson assumes no prior organic chemistry course has been taken.
Contents
Part 1 Microscopy 1 Brightfield Microscopy 2 Darkfield Microscopy 3 Phase-Contrast Microscopy 4 Microscopic Measurements Part 2 Survey of Microorganisms 5 Protozoa, Algae, and Cyanobacteria 6 Microscopic Invertebrates 7 Aseptic Technique 8 The Bacteria 9 Fungi: Yeasts and Molds Part 3 Microscope Slide Techniques 10 Negative Staining 11 Smear Preparation 12 Simple Staining 13 Capsular Staining 14 Gram Staining 15 Spore Staining: Two Methods 16 Acid-Fast Staining: Ziehl-Neelsen Method 17 Motility Determination Part 4 Culture Methods 18 Culture Media Preparation 19 Pure Culture Techniques 20 Cultivation of Anaerobes 21 Bacterial Population Counts 22 Slide Culture: Molds 23 Slide Culture: Autotrophs 24 Bacteriophage: Isolation and Culture Part 5 Environmental Influences and Control of Microbial Growth 25 Temperature: Effects on Growth 26 Temperature: Lethal Effects 27 pH and Microbial Growth 28 Osmotic Pressure and Bacterial Growth 29 Ultraviolet Light: Lethal Effects 30 Evaluation of Disinfectants: The Use-Dilution Method 31 Evaluation of Antiseptics: The Filter Paper Disk Method 32 Evaluaiton of Alcohol: Its Effectiveness as a Skin Degerming Agent 33 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method 34 Oligodynamic Action 35 Bacterial Mutagenicity and Carcinogenesis: The Ames Test 36 Effectiveness and Hand Scrubbing Part 6 Identification of Unknown Bacteria 37 Preparation and Care of Stock Cultures 38 Morphological Study of Unknown 39 Cultural Characteristics 40 Physiological Characteristics: Oxidation and Fermentation Tests 41 Physiological Characteristics: Hydrolytic Reactions 42 Physiological Characteristics: Miscellaneous Tests 43 Use of Bergey's Manual and Identibacter Interactus Part 7 Miniaturized Multitest Systems 44 Enterobacteriaceae Identification: The API 20E System 45 Enterobacteriaceae Identification: The Enterotube II System 46 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System 47 Staphylococcus Identification: The API Staph-Ident System Part 8 Microbiology of Water, Food, and Milk 48 Bacteriological Examination of Water Qualtative Tests 49 Bacteriological Examination of Water: The Membrane Filter Method 50 Standard Plate Count of Milk 51 Microbiology of Yogurt Production 52 Microbiology of Alcohol Fermentation 53 Bacterial Counts of Foods Part 9 Medical Microbiology and Immunology 54 A Synthetic Epidemic 55 The Staphylococci: Isolation and Identification 56 The Streptococci: Isolation and Identification 57 Gram-Negative Intestinal Pathogens 58 Urinary Tract Pathogens 59 Slide Agglutination: Serological Typing 60 Slide Agglutination (Latex) Test: For S. aureus Identification 61 Tube Agglutination Test: The Heterophile Antibody Test 62 Tube Agglutination: The Widal Test 63 Phage Typing 64 White Blood Cell Study: The Differential WBC Count 65 Blood Grouping 66 The Snyder Caries Susceptibility Test Appendix A Tables Appendix B Indicators, Stains, Reagents Appendix C Media Appendix D Identification Charts Appendix E The Streptococci Appendix F Identibacter interactus
