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Full Description
Legumes and oilseed crops are a rich source of protein and energy. The major objectives in grain-legume breeding are to increase grain yield, protein quantity and quality and digestibility, and to decrease toxic fac- tors, seed hardness and cooking time. Similarly, oilseed crops such as brassicas and peanut share somewhat similar genetic improvement goals, but suffer from susceptibility to a number of pathogens and diseases. In spite of the best efforts through conventional breeding, their yield has been virtually static, with no significant breakthrough. The lack of genetic variability in the base population has been considered to be a major limiting factor for the slow progress made in the improvement of these crops. The desired goals can be achieved by incorporating addi- tional genetic variability in the existing germplasm. The protein and oil contents which are genotypically oriented can be enhanced further by suitable crosses. In this regard, progress made during the last decade, in the area of in vitro manipulation and recombinant DNA technology, holds promise for the improvement of these crops. Among the oilseed crops, Brassica spp.
, soybean and sunflower have been well studied, wherein haploids, somaclones, somatic hybrids, cybrids and transformed plants have been produced. Oilpalm is one of the best examples where micropropagation is being commercially prac- ticed. Regarding legumes, though there is extensive work on forages, such as Medicago and Trifolium, much needs to be done on food legumes.
Contents
Section I Wide Hybridization, Transformation, Cryopreservation.- I.1 Wide Hybridization in Legumes and Oilseed Crops Through Embryo, Ovule, and Ovary Culture.- I.2 Transformation in Legumes.- I.3 Cryopreservation of Germplasm of Legumes and Oilseed Crops.- Section II Food Legumes.- II. 1 Pea (Pisum sativum L.).- II.2 Chickpea (Cicer arietinum L.).- II.3 Soybean: Plant Regeneration and Somaclonal Variation.- II.4 Soybean: Wide Hybridization Through Embryo Culture.- II.5 Soybean: Isolation, Culture, and Fusion of Protoplasts.- II.6 Winged Bean [Psophocarpus tetragonolobus (L.) D.C.].- Section III Forage Legumes.- III.1 Alfalfa, Lucerne (Medicago spp.).- III.2 Clovers (Trifolium spp.).- III.3 Lupins (Lupinus spp.).- III.4 Cell and Tissue Culture of Stylosanthes spp..- III.5 Birdsfoot Trefoil (Lotus corniculatus L.).- III.6 Crownvetch (Coronilla varia L.).- III.7 Goat's Rue (Galega officinalis L.).- Section IV Oilseed Crops.- IV. 1 Interspecific Hybridization in Brassica Through Ovary Culture.- IV.2 Regeneration of Plants from Protoplasts of Oilseed Brassica Crops.- IV.3 Cybrids in Oilseed Brassica Crops Through Protoplast Fusion.- IV.4 Sunflower (Helianthus annuus L.): Establishment of Cultures, Transformation, and the Regeneration of Plants.- IV.5 Sunflower (Helianthus annuus L.): In Vitro Interspecific Hybridization.- IV.6 In Vitro Production of Haploids in Helianthus.- IV.7 Sunflower (Helianthus spp.): Anther Culture and Field Studies on Haploids.- IV.8 Flax (Linum usitatissimum L.): In Vitro Studies.- IV.9 Flax (Linum usitatissimum L.): Embryo Culture Studies.- IV. 10 Coconut (Cocos nucifera L.): Micropropagation.- IV. 11 Coconut (Cocos nucifera L.): Anther Culture.- IV. 12 Oil Palm (Elaeis guineensis Jacq.): Tissue Culture and Micropropagation.- IV. 13 Olive (Oleaeuropaea L.) as an Oilseed Crop.- Section V Miscellaneous.- V.1 Indigofera spp..- V.2 Carob (Ceratonia siliqua L.).
