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Full Description
Yeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse. The simple nature of a single cell eukaryotic organism, the relative ease of manipulating its genome and the ability to interchangeably exist in both haploid and diploid states have always made it an attractive model organism. Genes can be deleted, mutated, engineered and tagged at will. Saccharomyces cerevisiae has played a major role in the elucidation of multiple conserved cellular processes including MAP kinase signaling, splicing, transcription and many others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Yeast Genetics: Methods and Protocols will provide a balanced blend of classic and more modern genetic methods relevant to a wide range of research areas and should be widely used as a reference in yeast labs.
Contents
Yeast Transformation by LiAc/SS Carrier DNA/PEG Method.- Tetrad, Random Spore and Molecular Analysis of Meiotic Segregation and Recombination.- PCR Mutagenesis and Gap Repair in Yeast.- PCR-Mediated Epitope Tagging of Genes in Yeast.- Manipulating the Yeast Genome: Deletion, Mutation and Tagging by PCR.- Preparation of Yeast Cells for Live Cell Imaging and Indirect Immunofluorescence.- Single Yeast Cell Imaging.- Microfluidic Platforms for Generating Dynamic Environmental Perturbations to Study the Responses of Single Yeast Cells.- Using Two-Hybrid Interactions to Identify Separation-of-Function Mutations.- Synthetic Genetic Array Analysis of Global Mapping of Genetic Networks in Yeast.- Chemical Genetic and Chemogenomic Analysis in Yeast.- Phenomic Assessment of Genetic Buffering by Kinetic Analysis of Cell Arrays.- Detection of Short-Range Chromatin Interactions by Chromosome Conformation Capture (3C) in Yeast.- Chromosome Conformation Capture (3C) of Tandem Arrays in Yeast.- Global Analysis of Transcription Factor Binding Sites in Yeast Using ChIP-Seq.- High-Density Tiling Microarrays Analysis of the Full Transcriptional Activity of Yeast.- Analysis of Silencing in Saccharomyces cerevisiae.- A User's Guide to the Ribosomal DANN in Saccharomysces cerevisiae.- Two-Dimensional Agarose Gel Electrophoresis for Analysis of DNA Replication.- Replicative Life Span Alaysis in Budding Yeast.- Metabolomic and Lipidomic Analyses of Chronologically Aging Yeast.
