- ホーム
- > 洋書
- > 英文書
- > Science / Mathematics
Full Description
Nucleases occupy a central position in the biochemistry of DNA transactions and other metabolism of nucleic acids in all organisms. They have also proven useful in modern biological studies crucial for the development of recombinant DNA technology and reverse genetics. Nucleases assist in the identification and characterization of genes responsible for several diseases and their possible alleviation by gene therapy. Molecular Biology of Nucleases introduces the properties and biological roles of nucleases. It is the one comprehensive source for newcomers to the field.
Contents
IntroductionHistorical PerspectivesProtein, RNA and Other Molecules as Enzymes and as NucleasesNature of Enzymatic Reactions Catalyzed by NucleasesClassificationNature of SubstrateMode of AttackSite SpecificityAssayGenetics of Nucleases and Biological RolesRibonucleaseGeneral RibonucleasesMicrobial NucleasesMammalian RibonucleasesPlant RibonucleasesEvolution of RibonucleasesRibonuclease Involved in RNA-ProcessingRNaseIIIRNasePRNaseERNaseM5RNaseDEukaryotic RNA Splicing EnzymesRibonuclease HE. coli RNaseHRetroviral Reverse Transcriptase RNaseHYeast RNaseHOther Eukaryotic RNaseHBiological Function of RNaseHProofreading Activity of RNA PolymeraseDeoxyribonucleaseClassification of EnzymesDeoxyribonucleasesEndonucleasesExonucleaseProperties of Enzymes from Different OrganismsBacterial EnzymesEndonucleasesRestriction EndonucleasesOccurrence, Classification, and Their General PropertiesDifferent Restriction Endonucleases and Their PropertiesType I Restriction EndonucleasesPurification and General PropertiesRecognition Sequences and Nature of SubstrateGeneticsCleavage MechanismType II Restriction EndonucleasesEnzyme Purification and AssayGeneral Properties of the EnzymesReaction Conditions and the Enzyme SpecificityNature of SubstrateInhibition of Restriction EndonucleasesRestriction Endonuclease GenesType III Restriction EndonucleasesEvolutionary Significance and Biological RoleDamage Specific NucleasesClassification and AssayAP EndonucleasesEnzymes that Directly Attack Phosphodiester Linkages in the Damaged DNA RegionAssayProperties of the Two Groups of Enzymes from Different OrganismsAP EndonucleasesAP Endonuclease Associated with Other Enzyme ActivitiesAP EndonucleasesDirect Acting EnzymesTopoisomerasesChoreography and Topology of DNAEnzyme AssayElectron MicroscopySedimentation MethodsAgarose Gel ElectrophoresisProperties of Enzymes from Different Groups of OrganismsProkaryotic TopoisomerasesEukaryotic TopoisomerasesMitochondrial TopoisomerasesViral TopoisomerasesGenetics and Biological RoleProkaryotic Topoisomerase MutantsEukaryotic Topoisomerase MutantsRecombinasesGeneral Description and ClassificationGeneral RecombinaseSite Specific RecombinaseTranspositional RecombinaseRNA RecombinaseProperties of Different RecombinasesGeneral RecombinaseSite Specific RecombinaseProkaryotic Site Specific RecombinaseEukaryotic Site Specific RecombinaseTranspositional RecombinaseControl of RecombinasesRNA RecombinaseSugar Non-Specific NucleasesGeneral Description, Classification, and Methods of AssayProperties of Enzymes from Different Groups of OrganismsMicrobial NucleasesAnimal NucleasesPlant NucleasesNon-Protein NucleasesRibozymesRNasePIntrons as RibozymesGroup II Intron RibozymesSplicosomal SnRNA RibozymeMaturaseHammerhead RNA as RibozymeCis- and Transacting Ribozyme EndonucleaseChemzymesChemicals and Metal Ligand Complexes as NucleasesPeptidesDesigner NucleaseMolecules that Interact with NucleasesInhibitorsProteins as Nuclease InhibitorsRNA as Nuclease InhibitorsOther Molecules that Act as Nuclease InhibitorsProteins that Interact with the Activity of Nuclease by Interacting with the Substrate (Nucleic Acids)DNA Sequences that Interact with NucleasesChi Like Elements in EukaryotesOther Inhibitor MoleculesProteins that Interact with DNA or Nuclease to Orchestrate the Activity of NucleasesBiological Function of NucleasesReplicationThree Steps in DNA ReplicationRole of Viral Nuclease in the Degradation of Host DNAInvolvement of Nuclease During the Separation of Daughter Helices at the End of ReplicationInvolvement of Nucleases in the Rolling Circle Mechanism of DNA ReplicationInvolvement of Nuclease in the Replication of Linear DNAInvolvement of Nuclease in the Replication of Chromosome in EukaryotesDNA RepairBaseless SitesSites with Altered Base or Incorrect BaseCrosslinking and Other DamagesDNA Repair MechanismsExcision RepairBypass Repair PathwaysRecombinational Repair PathwayInducible and Error Prone Repair PathwayMismatch RepairMismatch Repair in Mammalian CellsIncision of Damaged DNA Is a Complex Process Involving Several ProteinsExcision Repair Mutants of NeurosporaExcision Repair Mutants of YeastExcision Repair Mutants of DrosophilaExcision Repair Mutants of Mammalian CellsRecombinationDifferent Kinds of Genetic RecombinationRecombination Mechanisms and NucleasesGene Conversion and Postmeiotic SegregationIn Vitro Recombination SystemFungal Recombination NucleasesMismatch Repairs During RecombinationRecombination PathwaysRecombinational Control of Gene ExpressionRole of Recombinase in Mammalian Antibody Diversity, Allelic Exclusion , and Class SwitchT Cell Surface ReceptorEngineered Expression of Genes by RecombinaseDNA Transfection or TransformationMutationDNA Supercoiling and Maintenance of Chromosome StructureTranscriptionRNA ProcessingRNA TrimmingRNA SplicingRNA EditingControl of TranslationViral Maturation and EncapsidationNuclease in Defense MechanismNucleic Acid SalvageNucleases and Human DiseasesInvolvement of Nucleases in Human DiseaseXeroderma PigmentosumAxatia TelangiectasiaCockayne SyndromeCancerAgingImmunological DiseasesOther Human DiseasesNucleases and Neurological DisordersReverse Genetics, Human Diseases, and NucleasesUse of Nucleases in the Control of Human DiseasesNucleases as ToolsNature of "Transforming Principle" as DNAIsolation of DNA and RNANearest Neighborhood AnalysisIsolation of a GeneUniparental Transmission During Cytoplasmic InheritancePhysical Map of DNAUse of Nuclease in the Development of Recombinant DNA Technology and the Molecular Cloning of a GeneConstruction of an Artificial ChromosomeNew Method for Mapping Eukaryotic ChromosomesChromosome Walking (Overlap Hybridization)Role of Nucleases in Transposon MobilityUse of Nuclease in the Physical Mapping of a Mutational SiteBiological Activity of a DNA SegmentUse of Nucleases in the Identification of the Function of a DNA Segment Via Transformation ExperimentsUse of Nuclease in the Deletion Mapping of Biological ActivityUse of Nucleases in Identification of the Function of a DNA Segment via Marker Rescue MethodOrganization of Eukaryotic ChromosomesDistinction Between Active and Inactive Genes: The Relation Between Activity of a Gene and Nuclease Sensitive SiteDNA FootprintingConstruction of Mutants: Site Specific Mutation and Protein EngineeringNucleases in Directed MutagenesisNick Translation and Labeling of DNA with High Specificity RadioactivityRole of Nucleases in PCRGene KnockoutRNase Protection AssayUse of Nucleases in Forensic ScienceHuman Genome ProjectNucleases and EvolutionRibozyme as Evidence for the Early World of RNAChemzyme, Ribozyme, and ProteinzymeThe Role of Recombinase in EvolutionNucleases and Control of DNA Transactions and Their Roles in EvolutionRole of Nucleases in Directed Mutagenesis: Adaptive Mutation and SOS ResponseNucleases as Multifunctional MoleculesPossible Horizontal Transmission of Nuclease Gene and IntronConclusionsReferencesIndex
