- ホーム
- > 洋書
- > 英文書
- > Science / Mathematics
Full Description
High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC. Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.
Contents
INTRODUCTION, W.J. Lough and I.W. WainerAnalysis and ChromatographyHistorical Development of ChromatographyHPLC TodayRileyIntroductionChormatographic MobilityPeak ShapeRetention RelationshipsBand Broadening and Column EfficiencySeparationTerminologyChromatographic FormatModels of RetentionChoice of Mode of ChromatographyNormal-Phase Liquid ChromatographyReversed-Phased Liquid ChromatographyIon-Exchange ChromatographySize-Exclusion ChromatographySUPPORT MATERIALS AND SOLVENTS, P. HambletonAdsorption ChromatographyChemically Bonded SilicaPolymer PackingsPorous Graphitic CarbonSolvents for HPLCINSTRUMENTATION: PUMPS, INJECTORS, AND COLUMN DESIGN, T. NoctorIntroductionTypes of HPLC PumpApproaches to the Reduction of Flow PulsationThe Modern HPLC PumpValve InjectorsColumn DimemsionsColumn Inlet/OutletConnecting Tubing and UnionsConclusionINSTRUMENTATION: DETECTORS AND INTEGRATORS, D.K. LloydIdeal Detectors and Real DetectorsDetector Performance CriteriaThe UV-Visible Absorbance DetectorThe Fluorescence DetectorThe Refractive Index DetectorElectrochemical DetectorsOther Detection MethodsIntegratorsMETHOD DEVELOPMENT AND QUANTITATION, W.J. Lough and I.W. WainerMethod DevelopmentOptimisationRelative AssaysMethod ValidationTypes of Quantitative MethodSAMPLE PREPARATION, D. StevensonIntroductionSample Preparation ProceduresMatrix PropertiesAnalyte PropetiesExamples of ApplicationsAutomationConcluding RemarksPOLYMER ANALYSIS, A.J. HandleyIntroductionChromatography Modes Used for Polymer AnalysisSize Exclusion ChromatographyOther Modes of ChromatographyIntroductionBiomedical AnalysisForensic AnalysisENVIRONMENTAL ANALYSIS, P.J. RenneIntroductionHPLC ModeSample PreparationChoice of ColumnPumping SystemDetectorsSample IntroductionAnalytesMiscellaneousSuggested ProtocolConclusionsFOOD, ORGANIC, AND PHARMACEUTICAL APPLICATIONS, W.J. Lough and I.W. WainerIntroductionBulk SubstancesProducts or 'Formulations'Conclusion
