Laboratory Methods in Enzymology: DNA: Volume 529 (Methods in Enzymology") 〈529〉

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Laboratory Methods in Enzymology: DNA: Volume 529 (Methods in Enzymology") 〈529〉

  • オンデマンド(OD/POD)版です。キャンセルは承れません。
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  • 製本 Hardcover:ハードカバー版／ページ数 408 p.
  • 言語 ENG
  • 商品コード 9780124186873

Full Description


Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols.In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.

Contents

Explanatory chapterExplanatory Chapter: How Plasmid Preparation Kits WorkExplanatory Chapter: Introducing Exogenous DNA into cellsAgarose Gel ElectrophoresisAnalysis of DNA by Southern BlottingPurification of DNA Oligos by Denaturing Polyacrylamide Gel Electrophoresis (PAGE)Molecular CloningRapid creation of stable mammalian cell lines for regulated expression of proteins using the Gateway (R) Recombination Cloning Technology and Flp-In T-REx (R) linesRestrictionless cloningIsolation of plasmid DNA from bacteriaPreparation of Genomic DNA from BacteriaPreparation of Genomic DNA from Saccharomyces cerevisiaeIsolation of Genomic DNA from Mammalian CellsSanger Dideoxy Sequencing of DNAPreparation of fragment libraries for Next-Generation Sequencing on the Applied Biosystems SOLiD platformExplanatory Chapter: Next Generation SequencingGenerating mammalian stable cell lines by electroporationTransient mammalian cell Transfection with Polyethylenimine (PEI)Site-Directed MutagenesisPCR-based random mutagenesisMegaprimer Method for Mutagenesis of DNAExplanatory Chapter: Troubleshooting PCRExplanatory Chapter: Quantitative PCRGeneral PCRColony PCRChemical Transformation of YeastTransformation of E. coli via electroporationTransformation of Chemically Competent E. coli