基本説明
Contents: Characterisation; Labelling; Protocols: Preparation using SDS-PAGE; Measurement of Affinity using Friguet Method; Labelling of Antibodies with Fluorochrome; and more.
Full Description
This convenient lab manual gives quick and easy reference to key protocols and practical tips needed to undertake antibody applications. Thirty-five protocols are listed including a troubleshooting guide and tips to indicate hazards, good points to stop methods, key conditions, and reagent disposal.
Contents
INTRODUCTORY MATERIAL: Characterization of Antibodies; Labelling of Antibodies; Applications of Antibodies; PROTOCOLS: Antibody Concentration by Optical Density; Assessment of Purity of Antibody Preparation using SDS-PAGE; Measurement of Affinity using Friguet Method; Labelling of Antibodies with Biotin; Labelling of Antibodies with 125I; Labelling of Antibodies with Fluorochrome; Labelling of Antibodies with Enzyme; Direct ELISA; Sandwich ELISA; Competitive ELISA for Antigen Detection; Competitive ELISA for Epitope Mapping; ELISPOT; Fluid Phase Radioimmunoassay; Cell Surface Immunofluorescence; Intracellular Immunofluorescence on Cytospines; Intracellular Immunofluorescence on Cell Suspensions; Immunofluorescence on Tissue Sections; Enzyme-Linked Immunohistology; Immunoaffinity Chromatography using Columns; Batch Immunoaffinity Chromatography; Immunoprecipitation of Fluid Phase Antigen; Immunoprecipitation of Cellular Antigens; Ouchterlony Double Immunodiffusion; Single Radial Immunodiffusion.
